產品編號 | bs-23288R-HRP |
英文名稱1 | Rabbit Anti-KT3 tag/HRP Conjugated antibody |
中文名稱 | 辣根過氧化物酶標記的KT3 tag抗體 |
別 名 | KPPTPPPEPET Epitope Tag; KPPTPPPEPET Tag; KT3 Epitope Tag. |
規格價格 | 100ul/1200元 購買 大包裝/詢價 |
說 明 書 | 100ul |
產品類型 | 標簽抗體 |
研究領域 | 細胞生物 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Recombinant protein |
產品應用 | WB=1:10000-20000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide of KT3 tag |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | Constituents: 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Gentamicin, 50% glycerol. Or Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Gentamicin. Reconstitute with sterile distilled water. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產品介紹 |
background: The KT3 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized KT3 epitope represents the amino acid sequence KPPTPPPEPET derived from the Simian Virus 40 (SV40) large T-antigen. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |