產(chǎn)品編號(hào) | bs-0965R-Bio |
英文名稱1 | Rabbit Anti-FLAG Tag/Biotin Conjugated antibody |
中文名稱 | 生物素標(biāo)記的FLAG Tag標(biāo)簽抗體(N端) |
別 名 | DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag; DDDDK tag (N-T). |
規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
說(shuō) 明 書 | 100ul |
研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | |
產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | MAP of Flag Tag peptide C-terminus |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: This is a useful tool for the localisation and characterisation of DYKDDDDK tagged proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 蛋白質(zhì)純化的新途徑─應(yīng)用抗DYKDDDDK標(biāo)簽8肽抗體檢測(cè)DYKDDDDK標(biāo)簽N端8肽融合蛋白 1) DYKDDDDK標(biāo)簽8肽與不同的Sepharose純化填料結(jié)合(捆綁),可不受構(gòu)象的影響,在天然或變形的條件下可進(jìn)行進(jìn)一步純化。 2) 可在溫和的洗脫液環(huán)境中,其結(jié)合、洗滌及洗脫步驟不會(huì)對(duì)蛋白結(jié)構(gòu)產(chǎn)生影響。 3) DYKDDDDK標(biāo)簽比普通(Tag)要小得多,經(jīng)純化后的蛋白可直接進(jìn)行下游操作。可用于任何表達(dá)系統(tǒng)(包括: 細(xì)菌,桿狀病毒和哺乳動(dòng)物) 4) DYKDDDDK8肽標(biāo)簽在生理溶液pH下不帶電荷,其標(biāo)簽不會(huì)干涉重組蛋白的結(jié)構(gòu)和功能,不會(huì)影響蛋白分泌 5) DYKDDDDK8肽標(biāo)簽免疫原性差,重組蛋白可以不必除去標(biāo)簽而直接作為抗原進(jìn)行免疫。 |