| 產品編號 | bsm-33177M |
| 英文名稱 | Mouse Anti-TUBB3 (Neuronal Marker) antibody |
| 中文名稱 | 微管蛋白β3（神經標志物）單克隆抗體 |
| 別 名 | Tubulin beta 3; beta III Tubulin; Tubb3; Tubulin beta-3; beta 4; MC1R; TUBB 3; TUBB 4; TUBB3; TUBB4; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Neuron-specific class III beta-tubulin; Syntaxin III; Neuron specific beta III Tubulin; Tubulin beta-4 chain; Tubulin beta-III; beta-4; CDCBM; CFEOM3A; M(beta)3; M(beta)6; Neuron-specific class III beta-tubulin; QccE-11995; QccE-15186; TBB3_HUMAN; Tubulin beta 4; Tubulin beta-4 chain. TUJ1 |
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Specific References (3) | bsm-33177M has been referenced in 3 publications.
[IF=10.317] Yu D et al. MOF-encapsulated nanozyme enhanced siRNA combo: Control neural stem cell differentiation and ameliorate cognitive impairments in Alzheimer's disease model. Biomaterials
. 2020 Oct;255:120160. IHF&ICF ; Rat&Mouse.
[IF=5.923] Bing-Chun Liu. et al. Global Transcriptional Analyses of the Wnt-Induced Development of Neural Stem Cells from Human Pluripotent Stem Cells. Int J Mol Sci. 2021 Jan;22(14):7473 ICC ; Human.
[IF=3.046] Yuyuan Ma. et al. Ultra-structural morphology analysis of human cranial bone marrow mesenchymal stromal cells during neural differentiation. Neurosci Lett. 2021 Aug;:136179 ICC ; Human.
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| 研究領域 | 細胞生物 神經生物學 細胞類型標志物 細胞骨架 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 6F12 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-5000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TUBB3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Neuronal Marker Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Function: Tubulin is the major constituent of microtubules. Itbinds two moles of GTP, one at an exchangeable site on the betachain and one at a non-exchangeable site on the alpha chain. TUBB3plays a critical role in proper axon guidance and mantainance. Subunit: Dimer of alpha and beta chains. Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Expression is primarily restricted to centraland peripheral nervous system. Greatly increased expression in mostcancerous tissues. Post-translational modifications: Some glutamate residues at the C-terminus arepolyglutamylated. This modification occurs exclusively on glutamateresidues and results in polyglutamate chains on the gamma-carboxylgroup. Also monoglycylated but not polyglycylated due to theabsence of functional TTLL10 in human. Monoglycylation is mainlylimited to tubulin incorporated into axonemes (cilia and flagella)whereas glutamylation is prevalent in neuronal cells, centrioles,axonemes, and the mitotic spindle. Both modifications can coexiston the same protein on adjacent residues, and lowering glycylationlevels increases polyglutamylation, and reciprocally. The precisefunction of such modifications is still unclear but they regulatethe assembly and dynamics of axonemal microtubules (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, frommetaphase to telophase, but not in interphase. This phosphorylationinhibits tubulin incorporation into microtubules. DISEASE: Defects in TUBB3 are the cause of congenital fibrosis ofextraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenitalocular motility disorder marked by restrictive ophthalmoplegiaaffecting extraocular muscles innervated by the oculomotor and/ortrochlear nerves. It is clinically characterized by anchoring ofthe eyes in downward gaze, ptosis, and backward tilt of the head.Congenital fibrosis of extraocular muscles type 3 presents as anon-progressive, autosomal dominant disorder with variableexpression. Patients may be bilaterally or unilaterally affected,and their oculo-motility defects range from completeophthalmoplegia (with the eyes fixed in a hypo- and exotropicposition), to mild asymptomatic restrictions of ocular movement.Ptosis, refractive error, amblyopia, and compensatory headpositions are associated with the more severe forms of thedisorder. In some cases the ocular phenotype is accompanied byadditional features including developmental delay, corpus callosumagenesis, basal ganglia dysmorphism, facial weakness,polyneuropathy. Defects in TUBB3 are the cause of cortical dysplasiacomplex with other brain malformations (CDCBM) [MIM:614039]. CDCBMis a disorder of aberrant neuronal migration and disturbed axonalguidance. Affected individuals have mild to severe mentalretardation, strabismus, axial hypotonia, and spasticity. Brainimaging shows variable malformations of cortical development,including polymicrogyria, gyral disorganization, and fusion of thebasal ganglia, as well as thin corpus callosum, hypoplasticbrainstem, and dysplastic cerebellar vermis. Extraocular musclesare not involved. Similarity: Belongs to the tubulin family. SWISS: Q13509 Gene ID: 10381 Database links: Entrez Gene: 10381 Human Entrez Gene: 22152 Mouse Omim: 602661 Human SwissProt: Q13509 Human SwissProt: Q9ERD7 Mouse Unigene: 511743 Human Unigene: 40068 Mouse Unigene: 43958 Rat |
| 產品圖片 |
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- TUBB3 (bsm-33177M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Lane 1: Human A549 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human U251 cell lysates
Lane 4: Human U87MG cell lysates
Primary: Anti- TUBB3 (bsm-33177M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 47 kDa
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti- TUBB3 (bsm-33177M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUBB3) Polyclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TUBB3 (Neuronal Marker) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
