| 產(chǎn)品編號 | bs-6988R |
| 英文名稱 | Rabbit Anti-RuBisCO antibody |
| 中文名稱 | 核酮糖1,5二磷酸羧化酶/二磷酸核酮糖鹽羧化酶大鏈抗體 |
| 別 名 | rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; RuBisCO-large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) [Arabidopsis lyrata]. |
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Specific References (3) | bs-6988R has been referenced in 3 publications.
[IF=5.36] Bohley K et al. C4-like photosynthesis and the effects of leaf senescence on C4-like physiology in Sesuvium sesuvioides (Aizoaceae). J Exp Bot. 2019 Mar 11;70(5):1553-1565. WB ; Sesuvium sesuvioides.
[IF=3.59] Zhang, Tai-Jie, et al. "A magic red coat on the surface of young leaves: anthocyanins distributed in trichome layer protect Castanopsis fissa leaves from photoinhibition." Tree Physiology (2016). WB ; Other Species.
[IF=2.632] Zhang Q et al. The Changing Distribution of Anthocyanin in Mikania micrantha Leaves as an Adaption to Low-Temperature Environments. Plants (Basel). 2019 Oct 27;8(11). pii: E456. WB ; M. micrantha.
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| 產(chǎn)品類型 | 植物抗體 |
| 研究領域 | 腫瘤 細胞生物 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Arabidopsis Thaliana) |
| 產(chǎn)品應用 | WB=1:500-1000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana Rubisco: 151-250/479 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. Function: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. Subunit: Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers. Subcellular Location: Plastid, chloroplast. Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover. The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization. Similarity: Belongs to the RuBisCO large chain family. Type I subfamily. SWISS: P00875 Gene ID: 2715621 Database links: Entrez Gene: 2715621 Spinacia oleracea SwissProt: P00875 Spinacia oleracea |
| 產(chǎn)品圖片 |
Sample: Linden Lysate at 40 ug
Primary: Anti-RUBISCO (bs-6988R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
Sample:
Gourd leaf Lysate at 40 ug
Lotus leaf Lysate at 40 ug
Primary: Anti-RUBISCO (bs-6988R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
