| 產(chǎn)品編號 | bs-0514P |
| 英文名稱 | Recombinant human BMP-2 protein, N-His |
| 中文名稱 | 重組人骨形態(tài)發(fā)生蛋白-2 |
| 別 名 | bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2; BMP2A; BMP-2; BMP2_HUMAN; Recombinant human BMP-2 protein, N-His |
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Specific References (4) | bs-0514P has been referenced in 4 publications.
[IF=8.31] Li, Long, et al. "Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect." Biomaterials (2014). other ;
[IF=5.295] Jing Liu. et al. Protection of primary cilia is an effective countermeasure against the impairment of osteoblast function induced by simulated microgravity. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2022 Dec;:
[IF=5.039] Wu Z et al. The synergetic effect of bioactive molecule-loaded electrospun core-shell fibres for reconstruction of critical-sized calvarial bone defect-The effect of synergetic release on bone Formation. Cell Prolif. 2020 Apr;53(4):e12796. other ;
[IF=3.192] Zhou XH et al. In vitro and in vivo anti-corrosion properties and bio-compatibility of 5β-TCP/Mg-3Zn scaffold coated with dopamine-gelatin composite. Surface and Coatings Technology. 2019 doi:10.1016/j.surfcoat.2019.05.046 Other ;
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| 理論分子量 | 13kDa |
| 檢測分子量 | 17 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | hu, mo, rat, dog, pig, cow,Rb |
| 序 列 | 283-396/396 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內(nèi)毒素 | Not analyzed |
| 表達(dá)系統(tǒng) | E.coli |
| 活性 | Yes |
| 標(biāo)簽 | N-His |
| 緩 沖 液 | 20mM Tris-HCl (pH8.0). |
| 保存條件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis. SWISS: P12643 Gene ID: 650 骨形態(tài)發(fā)生蛋白-2 骨組織形成(rh-BMP-2)是一個(gè)由多種因素調(diào)節(jié)的復(fù)雜而有序的過程。在細(xì)胞水平上看，骨組織形成有兩種方式，即膜內(nèi)成骨和軟骨內(nèi)成骨。前者的新生骨組織在結(jié)締組織膜中由間充質(zhì)細(xì)胞直接形成，后者是指結(jié)締組織內(nèi)的間充質(zhì)細(xì)胞首行先分化為軟骨組織，然后在軟骨組織的基礎(chǔ)上進(jìn)行鈣化成骨。 從分子水平上看，骨組織的形成過程就是生物大分子在細(xì)胞與細(xì)胞之間、細(xì)胞與細(xì)胞外基質(zhì)之間的信息轉(zhuǎn)導(dǎo)過程。 胚胎時(shí)期的骨化過程同骨折骨痂的形成以及異位骨形成過程相類似，均受到機(jī)體某種激素和某些局部生長因子的調(diào)控。 一般認(rèn)為:1.影響骨組織形成的激素主要有： 甲狀旁腺素(PTH)、生長素、甲狀腺素、降鈣素、雌激素等； 2.影響骨組織形成的局部生長因子主要有： BMPs、轉(zhuǎn)移因子(TGF)、血小板衍生生長因子(PDGF)、胰島素樣生長因子(IGFs)、白細(xì)胞介素(ILs)等。 在骨組織形成過程中，激素與生長因子調(diào)節(jié)之間既有相對獨(dú)立，又相互聯(lián)系。兩種調(diào)節(jié)與神經(jīng)系統(tǒng)共同形成了神?內(nèi)分泌-免疫-局部生長因子調(diào)節(jié)網(wǎng)絡(luò)，參與骨組織形成的整個(gè)調(diào)節(jié)過程。一般認(rèn)為，局部生長因子在骨組織形成的調(diào)節(jié)過程中起著主導(dǎo)作用。尤其BMPs是其中唯一能夠單獨(dú)誘導(dǎo)間充質(zhì)細(xì)胞向骨組織方向分化的生長因子，是骨組織形成過程中最關(guān)鍵的調(diào)節(jié)因子。 實(shí)驗(yàn)表明，低濃度BMPs能夠誘導(dǎo)間充質(zhì)細(xì)胞向骨組織形成區(qū)移行；中等濃度BMPs可以促進(jìn)間充質(zhì)細(xì)胞向成軟骨及成骨細(xì)胞方向分化；而高濃度BMPs則能促進(jìn)進(jìn)間充質(zhì)細(xì)胞的增生。 研究認(rèn)為，BMP-2主要對未分化間充質(zhì)細(xì)胞和骨系細(xì)胞起到募集和分化作用。在骨形成早期，BMP-2不僅可使未分化間質(zhì)細(xì)胞向骨形成中心募集，并分化為骨系細(xì)胞，而且可使成纖維細(xì)胞、成肌細(xì)胞及骨髓的基細(xì)胞逆轉(zhuǎn)分化為骨系細(xì)胞。其主要過程是：增加或抑制這些細(xì)胞內(nèi)的某些特異性蛋白的分泌，使成纖維細(xì)胞分化為成骨細(xì)胞，成肌細(xì)胞快速分化為肥大的軟骨細(xì)胞，并促進(jìn)基質(zhì)鈣化。 對于成骨細(xì)胞，BMP-2則可使之維持其特有細(xì)胞表型，并誘導(dǎo)成骨細(xì)胞標(biāo)志物的增高，促進(jìn)細(xì)胞外基質(zhì)鈣化。在骨形成后期，BMP-2還作為一種破骨細(xì)胞分化因子與其它支持破骨細(xì)胞分化因子直接或間接刺激破骨細(xì)胞分化，參與骨的重建。 關(guān)于BMP-2是否具有促進(jìn)細(xì)胞的有絲分裂和復(fù)制作用，目前尚有爭議。但可以肯定，在促進(jìn)細(xì)胞有絲分裂和復(fù)制過程中，BMP-2與其它局部因子有協(xié)同作用。因此骨組織形成是多種局部因子共同調(diào)節(jié)的結(jié)果. |
| 產(chǎn)品圖片 |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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