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Rabbit Anti-PTHLH  antibody (bs-1107R)
~~~促銷代碼KT202410~~~
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產(chǎn)品編號(hào) bs-1107R
英文名稱 Rabbit Anti-PTHLH  antibody
中文名稱 甲狀旁腺激素相關(guān)蛋白抗體
別    名 Parathyroid Hormone Related Protein; Parathyroid Hormone-related Protein; HHM; MGC14611; Osteostatin; Parathyroid hormon like hormone isoform 2 preproprotein; Parathyroid hormone like hormone; Parathyroid hormone like hormone isoform 1 preproprotein; Parathyroid hormone like protein; Parathyroid hormone like related protein; Parathyroid Hormone Receptor 1; Parathyroid like protein; PLP; PTH related protein; PTH rP; PTHrP; PTHR; PTHRP.  
Specific References  (1)     |     bs-1107R has been referenced in 1 publications.
[IF=3.36] Zhao L et al. Reducing macrophage numbers alleviates temporomandibular joint ankylosis. Cell Tissue Res. 2019 Sep 14.  IHC ;  Human.  
研究領(lǐng)域 免疫學(xué)  生長因子和激素  內(nèi)分泌病  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 16kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PTHLH: 101-177/177 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Parathyroid related protein (PTHLH), is a polypeptide hormone produced by almost every tissue of the body and is closely related to parathyroid hormone (PTH). It signals through its receptor, PTHR1, regulating endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. The receptor for this hormone (PTHRP) is responsible for most cases of humoral hypercalcemia of malignancy.

Function:
This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

Subunit:
PTHrP interacts with PTH1R (via N-terminal extracellular domain).

Subcellular Location:
Cytoplasmic, Nuclear and Secreted

Tissue Specificity:
Expressed in most tissues. Most abundant in kidney, bone and liver.

DISEASE:
Defects in PTH1R are the cause of Jansen metaphyseal chondrodysplasia (JMC) [MIM:156400]. JMC is a rare autosomal dominant disorder characterized by a short-limbed dwarfism associated with hypercalcemia and normal or low serum concentrations of the two parathyroid hormones.
Defects in PTH1R are the cause of chondrodysplasia Blomstrand type (BOCD) [MIM:215045]. BOCD is a severe skeletal dysplasia. Defects in PTH1R may be a cause of enchondromatosis multiple (ENCHOM) [MIM:166000]. Enchondromas are common benign cartilage tumors of bone. They can occur as solitary lesions or as multiple lesions in enchondromatosis (Ollier and Maffucci diseases). Clinical problems caused by enchondromas include skeletal deformity and the potential for malignant change to osteosarcoma.

Similarity:
Belongs to the G-protein coupled receptor 2 family.

SWISS:
P12272

Gene ID:
5744

Database links:

Entrez Gene: 5744 Human

Entrez Gene: 19227 Mouse

Entrez Gene: 24695 Rat

Omim: 168470 Human

SwissProt: P12272 Human

SwissProt: P22858 Mouse

SwissProt: Q540C1 Mouse

SwissProt: Q811S6 Mouse

SwissProt: Q924X4 Mouse

SwissProt: P13085 Rat

Unigene: 89626 Human

Unigene: 9750 Rat



產(chǎn)品圖片
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PTHLH Polyclonal Antibody, Unconjugated(bs-1107R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PTHLH Polyclonal Antibody, Unconjugated(bs-1107R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PTHrP/PTHLH Polyclonal Antibody, Unconjugated(bs-1107R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:A549. Primary Antibody (green line): Rabbit Anti-PTHLH antibody (bs-1107R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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