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Rabbit Anti-Calretinin  antibody (bs-0062R)
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產(chǎn)品編號 bs-0062R
英文名稱 Rabbit Anti-Calretinin  antibody
中文名稱 鈣結(jié)合蛋白抗體
別    名 29 kDa calbindin; CAB 29; CAB29; CAL 2; CAL2; CALB 2; CALB2; Calbindin 2 29kDa; Calbindin 2; Calbindin D29K; Calbindin2; CR; CALB2_HUMAN; Calretinin.  
Specific References  (1)     |     bs-0062R has been referenced in 1 publications.
[IF=3.9] Suzui, Masumi, et al. "Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors." Cancer Science 107.7 (2016): 924-935.  IHC-P ;  Rat.  
研究領(lǐng)域 神經(jīng)生物學(xué)  結(jié)合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Rat (predicted: Mouse,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 29kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Calretinin: 211-271/271 
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Calretinin is a calcium-binding protein which is abundant in auditory neurons. It belongs to the calbindin family. Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin1.

Function:
Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Tissue Specificity:
Brain.

Similarity:
Belongs to the calbindin family.
Contains 6 EF-hand domains.

SWISS:
P22676

Gene ID:
794

Database links:

Entrez Gene: 396255 Chicken

Entrez Gene: 794 Human

Entrez Gene: 12308 Mouse

Entrez Gene: 117059 Rat

Entrez Gene: 393684 Zebrafish

Omim: 114051 Human

SwissProt: P07090 Chicken

SwissProt: P22676 Human

SwissProt: Q08331 Mouse

SwissProt: P47728 Rat

Unigene: 106857 Human

Unigene: 2755 Mouse

Unigene: 10321 Rat




鈣結(jié)合蛋白(Calretinin,CR)主要存在于神經(jīng)組織中,近10%的腺癌可以表達(dá)此蛋白的局部染色陽性,而絕大多數(shù)的間皮瘤為陽性表達(dá),因此它是診斷間皮瘤的一個(gè)有用的標(biāo)記物 。
鈣結(jié)合蛋白是平滑肌和非肌性調(diào)控蛋白。它可以與肌動蛋白、肌球蛋白、原肌球蛋白和鈣調(diào)素相互作用。
產(chǎn)品圖片
Sample: Lane 1: Mouse Cerebrum Lysates Lane 2: Mouse Cerebellum Lysates Lane 3: Rat Cerebrum Lysates Primary: Anti- Calretinin (bs-0062R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
Sample: U-87MG(Human) Cell Lysate at 30 ug Primary: Anti-Calretinin (bs-0062R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Calretinin/CA Polyclonal Antibody, Unconjugated(bs-0062R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:A431. Primary Antibody (green line): Rabbit Anti-S100A13 antibody (bs-2617R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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